This is the first time the Impala is seen with Ohio license plates instead of Kansas plates. Sam explains this was done due to their fugitive status.
It bears a Sedgwick County, Kansas license (though their hometown of Lawrence, Kansas is actually in Douglas County) plate KAZ 2Y5, a reference to Kansas, the Winchesters' home state, and 2005, the year the show premiered. Starting in What Is And What Should Never Be, the car sports a new Ohio license plate (CNK 80Q3).
